README File: Title: Paper-based nanosensors to evaluate community-wide illicit drug use for wastewater-based epidemiology Authors: KangMao, ZhugenYang, HuaZhang, XiqingLi, Jonathan M.Cooper This readme file aims to provide a guide to use the data associated with this publication, available here in the following folders : 1. data of figure 1 2. data for figure 3 2.1 Figure 3. (a) SERS spectra of CV (100 nM) acquired from 8 different SERS substrates within a SERS array, showing a high degree of reproducibility 2.2 Figure 3. (b) SERS spectra (λex = 785 nm) of CV (100 nM) acquired from a freshly prepared Au@Ag-pLL-GNFP SERS substrate (No. 3) and from a substrate prepared one month (No. 2) and two months (No. 1) earlier, showing good long-term stability of the nanofibrous paper-Au@Ag assembly. 3. data of Figure 4 3.1 Figure 4. SERS spectra of methamphetamine measured on Au@Ag-modified glass nanofibrous paper substrate. 3.2 The inset shows the SERS spectra from DFT simulations of Figure 4. 4. raw data of Figure 5 4.1 Figure 5. SERS assay performance of different concentrations of (a) methamphetamine (0.01 to 10000 ppb) on Au@Ag-pLL assembled on the glass nanofibrous paper substrate. 4.2 Figure 5. (b) Standard curve of the corresponding peak intensities at 1016 cm−1 and ΔI1016 as a function of drug concentration. 5. Raw data of Figure 6 5.1 Figure 6. (a) Detection of 100 ppb methamphetamine in different matrices (buffer, lake and river water) using the SERS sensors (black) and LC-MS (white) as the gold-standard reference method. 5.2 Figure 6(b) normalized methamphetamine concentrations in wastewater treatment plants measured using the SERS sensor (white) and HPLC-MS/MS (black) 5.3 Figure 6(c) normalized concentrations of methamphetamine determined by the SERS sensor in influent (black) and effluent (white) from wastewater treatment plants in Beijing, demonstrating the effectiveness of the wastewater treatment process. ESI 6. data of Figure S1. UV-vis spectra of Au@Ag (red line) and AuNPs (black line).